Beta thalassemia is one of the commonest recessive genetic diseases. It results in a severe anemia needing monthly transfusion. Our aim was to evaluate hematological profile of cord blood HPLC for prenatal diagnosis of beta thalassemia. Cord blood samples at 18-24 weeks gestation from 113 mothers suspected for beta thalassemia was studied. Out of them 34 was maternal contamination. HbF& HbA0 were determined by cation exchange high performance liquid chromatography. In HPLC studies for beta thalassemia, 13 fetuses were affected, 32 were carriers and 34 were normal.