Applied Cell Biology

Applied Cell Biology

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Protective Effect of the QiBracelet® Against Oxidative Stress

Peter C. Dartsch*

Dartsch Scientific GmbH, Institute for Cell Biological Test Systems, Auf der Vosshardt 25, D-49419 Wagenfeld, Germany

Abstract

Environmental oxidative stress is caused by different artificial and natural sources (environmental stressors). These influences might increase the generation of reactive oxygen species which are known to cause unwanted adverse health effects. According to the manufacturer, the QiBracelet® contains a grid chip which forms a static field that stimulates water molecules to undergo a transition into the coherent state. Since our body consists of about 70-85 % of water, the coherent state of the water molecules should be able to influence the cells of our whole body in a positive manner. Thus, the cells might obtain the ability to compensate any environmental stressors causing oxidative stress much more efficient when compared with endogenous enzymatic systems.

The goal of this study was to investigate whether different cultured cell types were able to resist exogenous oxidative stress by addition of 2 mM hydrogen peroxide to the culture medium much more efficient in the presence of the QiBracelet® in comparison to unprotected control cells.

Without protection, connective tissue fibroblasts (L-929) were the most sensitive cell type, while lung cells (A-549) were the least sensitive; connective tissue fibroblasts showed a loss in viability of 24% and lung cells of 84%. The other cell types such as kidney cells (MDCK), liver cells (Hep G2) and intestinal epithelial cells (IPEC-J2) had a viability between the two values. Independent from cell type, the QiBracelet® demonstrated a protective effect against exogenous oxidative stress. The extent of this protection varied among cell types, with liver cells showing the highest level of protection at 47.3 ± 7.1 %, followed by connective tissue fibroblasts at 29.6 ± 5.6%, kidney cells at 27.1 ± 5.9%, intestinal epithelial cells at 18.0 ± 7.1%, and lung cells at only 3.9 ± 2.8%.

The results demonstrate that the use of the QiBracelet® was able to protect all tested cell types from the hydrogen peroxide-induced environmental oxidative stress. Therefore, the QiBracelet® might also promote and maintain a better well-being and attitude of life in vivo.

Keywords:
Oxidative stress; Coherent water; MDCK; Hep G2; IPEC-J2; L-929; A-549; Cell viability; Cell culture
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