Applied Cell Biology

Temporal variations in trace element profile in different segments of fallopian tube during oestrous cycle in Caprines

R.K.Sharma, R.Singh, J.K.Bhardwaj

Reproductive PhysiologyLaboratory,Department ofZoology,KurukshetraUniversity, Kurukshetra-136119Haryana, (INDIA)

Abstract

Variations in secretory activity and macromolecular fluctuations in different segments of oviduct during oestrous cycle have been recorded however no information is available on changes in microminerals concentration of goat oviduct till date. Therefore, the quantitative analysis of different segments of the fallopian tube i.e. infundibulum, ampulla and isthmus of goat (Capra hircus) was conducted to study the alterations in trace elements viz iron (Fe), zinc (Zn), copper (Cu) and manganese (Mn) during the follicular and luteal phases. The iron content (mean±SEM) decreased frominfundibulumto isthmus (0.97±0.20, 0.74±0.14, 0.61±0.06 μg/100 mg respectively) during the follicular phase and increased frominfundibulum to isthmus (0.83±0.18, 1.00±0.14, 1.28±0.06 μg/100 mg respectively) during the luteal phase. The iron content was maximum in the isthmus and ampulla during the luteal phase. Similarly, zinc content was also higher in the isthmus and ampulla of the luteal phase. The copper content in the oviductal segments was high in the luteal phase as compared to the follicular phase. The manganese content declined from infundibulum to isthmus during the follicular phase. The present study revealed the pattern of changes in micro mineral concentration of the oviduct that maintains and modulates a milieu crucial for early developmental events. The results of the present findings will help in formulation of specificmedia contents for in vitro maturation of gametes, fertilization and early embryo development.

Keywords:

Trace elements; Fallopian tube; Caprine; Oestrous cycle.