Applied Cell Biology

Isolation of actinobacteria and its antagonistic effects on native organisms from Northern black soils of Katheru (A.P), India

Suseela Lanka, Pydipalli Muralidhar, T.Venkata Narsaiah, J.Naveena Lavanya Latha

¹Department ofBiotechnology,KrishnaUniversity,Machilipatnam,Andhra Pradesh, (INDIA)
²VIMTALABS,Cherlapally,Hyderabad, (INDIA)

Abstract

Actinobacteria are a group of prokaryotic organisms belonging to Grampositive bacteria and play an important ecological role in recycling substances in the nature. The main objective of the present study was to isolate and identifyActinobacteria from Northern black soils of Katheru, Rajahmundry district, Andhra Pradesh, INDIA, where tobacco is being cultivated on a large scale. The isolates were identified usingmorphological characters and identified as Streptomyces. Further the isolates were examined for antimicrobial activity. The results obtained indicate that Streptomyces II possess positive antagonistic effect on both gram positive
Bacilli and Cocci, where as Streptomyces I possess negative antagonistic effect. They were further tested for their antagonistic effect on different fungi viz., Aspergillus flavus, Aspergillus niger, Pythium, Rhizoctonia solani and Sclerotium. Streptomyces II showed antagonistic effect on all fungi except Sclerotium where as Streptomyces I showed negative antagonistic effect.  Biochemical studies revealed that both species of Streptomyces are amylase positive. Thus, Streptomyces II may be used for the production of polymeric anti-fungal antibiotics and to treat some of bacterial diseases of tobacco.

Keywords:

Actinobacteria; Antagonist; Bacilli; Cocci; Antibiotics.