Histological and flow cytometrical effects of white tea extract against mercury-induced hepatotoxicity in mice
Eman Mohamed Hussine, Attalla Farag El-Kott
1ZoologyDept.,Faculty of Science, FayoumUniversity, (EGYPT)
2ZoologyDept.,Faculty of Science,DamanhourUniversity, (EGYPT)
Liver plays an important and pivotal role in transforming and remove chemicals that may cause the hepatotoxicity such as heavy metals especially mercury. The protective effect of white tea extract against the hepatotoxicity induced by HgCl2 in mice was studied. Biochemical, histopathological and flowcytometrical investigations were estimated. The animals were divided into four groups, each of 8 animals: Group 1 was served as the control group, themice were injected i.p.with saline (1ml/ day). In group 2, mice were orally administered with freshly prepared aqueous extract of white tea (100 mg / kg / day). Animals of group 3 were i.p. injected with HgCl2 (1mg/ kg / day).Animals in group 4 were injected i.p for 14 dayswith HgCl2 then administered with white tea extract for another 14 days. The AspartateAminotransferase (AST),AlanineAminotransferase (ALT) levels andAlkaline phosphatase (ALP) level are significantly lower (P<0.05) in the mice of the control group than both mice in groups 3 and 4. Liver histopathological showed that white tea extract reduced fatty degeneration, cytoplasmic vacuolation and necrosis in HgCl2-treated mice. The significant increases in apoptotic cells were observed after the animals exposed to HgCl2 and decreases in the group exposed to HgCl2 and treated with white tea extract. This study suggests that white tea possesses antioxidant, anti-toxic and anti-apoptotic properties effects in the hepatoprotective effect against mercury toxicity.
White tea; Mercuric chloride; Hepatotoxicity; Flowcytometry.