Expression of genes, which participate in the control cell proliferation, in subcutaneous adiposet issue of the obese men with glucose intolerance
Oleksandr H.Minchenko, Yulia M.Bashta, Dmytro O.Minchenko, Olena V.Kovalevska, Anna O.Kulinich
1Department ofMolecularBiology, Palladin Institute ofBiochemistry,NationalAcademy of Sciences ofUkraine, Kyiv 01601, (UKRAINE)
The development of obesityand itsmetabolic complications, the most profound public health problems, is associated with dysregulation of different intrinsic mechanisms which control most basic metabolic processes. We have studied the expression levels ofmRNAofCTGF,MYLK,MEST, PLAU, PLAT, PLAUR, SERPINE1, TPD52, ITGA3, ITGB1, ITGAM, and ITGAVfactorsrelated to the control of proliferation processes in subcutaneous adipose tissueof obese menwith and withoutimpaired glucose tolerance. It was shown that the expression levelall of these genes significantly increase in subcutaneous adipose tissue in obese menwithout impaired glucose tolerance as compared tocontrol group of lean subjects. Magnitude of obesity-induced changes in expression levels of different factorswas gene specific and more robust in the case of CTGF andSERPINE1 genes. Further increase of the expression levels of PLAU, PLAUR, SERPINE1, ITGA3, andITGAV mRNAsin subcutaneous adipose tissue was found in obese patients with impaired glucose tolerance.At the same time, development of glucose intolerance in obese individuals leads to down-regulation of the expression of genes which encode forCTGF, MYLK, PLAT, and TPD52. Results of this study provide strong evidence that expression of genes mostly related to the regulation of proliferationisup-regulated in adipose tissue of obese individuals and possiblycontribute to fat tissue storage. Glucose intolerance-associated changes in expression levels of mRNA of studied genesin obese patients were more prominent in the case of CTGF, PLAUR, and SERPINE1 and possibly contribute to the development of obesity complications.