Applied Cell Biology

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EBN Lower Oxidative Stress: Lactoferrin and Ovotransferrin Contribute toward Antioxidative Effects of Edible Bird’s Nest Against Hydrogen Peroxide-Induced Oxidative Stress in Human SH-SY5Y Cells

Hou Zhiping, Mustapha Umar Imam, Maznah Ismail, Nur Hanisah Azmi, Norsharina Ismail, Aini Ideris4 and Rozi Mahmud

1Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia
2Department of Nutrition and Dietetics, Universiti Putra Malaysia, Selangor, Malaysia
3Department of Pathology, Chengde Medical University, Hebei, China
4Department of Veterinary Clinical Studies, Veterinary Medicine Faculty, Universiti Putra Malaysia, Selangor, Malaysia
5Department of Imaging, Medicine and Health Science Faculty, Universiti Putra Malaysia, Selangor, Malaysia


There are reports of improved redox outcomes due to consumption of Edible Bird’s nest (EBN). Many of the functional effects of EBN can be linked to its high amounts of antioxidants. Interestingly, dietary components with high antioxidants have shown promise in the prevention of aging and its related diseases like Alzheimer’s disease. In this study, the antioxidative potentials of EBN and its constituents, Lactoferrin (LF) and Ovotransferrin (OVF), were determined and protective effects against H2O2-induced toxicity on SH-SY5Y cells using MTT (3-(4,5-Dimethylthiazol-2-yl)- 2,5-Diphenyltetrazolium Bromide) assay and acridine orange and propidium iodide (AO/PI) staining with microscopy. Results showed that EBN and its constituent’s attenuated hydrogen peroxide (H2O2) induced cytotoxicity and decreased radical oxygen species (ROS) through increased scavenging activity. Furthermore, LF, OVF and EBN produced transcriptional changes in anti-oxidant related genes that tended towards neuroprotection as compared to H2O2 treated group. Overall, the results suggest that LF and OVF may produce synergistic or all-or-none anti-oxidative effects in EBN.

Edible Bird’s Nest; lactoferrin; Ovotransferrin; Antioxidant; Neuroprotection
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