Applied Cell Biology
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Cis-9. trans-11 conjugated linoleic acid against lipid peroxidation of rat brain homogenate andmitochondria
A.Palacios, A.Terrasa
Cátedr a de Bioquímica, F acultad de Ciencias Veter inar ias, Univer sidad Nacional de L a Plata, (ARGENTINA)
Abstract
In the present study it was evaluated the in vitro non-enzymatic lipid peroxidation of brain homogenate and mitochondria of two groups of Wistar rats: control and c9, t11 conjugated linoleic acid -supplemented. Lipid peroxidation was monitored by chemiluminescence and changes in the fatty acid composition byGLC.After incubation of brain homogenates and mitochondria in an ascorbate-Fe2+ system, it was observed that the total cpm originated from light emission, was lower, with no significant statistical differences, in those preparations isolated from CLA-supplemented group than in the control group The fatty acid composition was substantially modified during the lipid peroxidation with considerable decrease of polyunsaturated fatty acids, arachidonic acid C20:4! 6 and docosahexaenoic acid C22:6! 3 (PUFAs). In the control group the homogenate and mitochondria PUFAs contents diminished from 24.09 ± 1.35 to 10.76 ± 2.74 and 25.13 ± 4.34 to 12.25 ± 1.48%, respectively. No significant differences were observed when CLA group was compared to the control group. We conclude that in our experimental conditions, CLA did not protect the PUFAs from oxidative damage in the brain.
