Applied Cell Biology

Carrier detection of â thalassaemia: An emerging role of HPLC

S.Pandey, R.Saxena, R.M.Mishra, U.K.Chauhan, M.Sharma, Sw.Pandey

¹Dept ofHematology,All India Institute ofMedical Sciences,AnsariNagar, New Delhi - 110 029, (INDIA)
²Dept ofEnvironmentalBiology,APSUniversity,Rewa - 486003, (INDIA)
³Centre for Biotechnology Studies,APSUniversity,Rewa - 486003, (INDIA)
⁴Dept ofHematology, SafdarjungHospital,AnsariNagar,New Delhi - 110029, (INDIA)

Abstract

Beta thalassemia is one of the commonest recessive genetic diseases. It results in a severe anemia needing monthly transfusion. Our aim was to evaluate hematological profile of cord blood HPLC for prenatal diagnosis of beta thalassemia. Cord blood samples at 18-24 weeks gestation from 113 mothers suspected for beta thalassemia was studied. Out of them 34 was maternal contamination. HbF& HbA0 were determined by cation exchange high performance liquid chromatography. In HPLC studies for beta thalassemia, 13 fetuses were affected, 32 were carriers and 34 were normal.

Keywords:

Cord blood; HPLC; Beta thalassemia.