Applied Cell Biology

Applied Cell Biology

E-mail: editor@appliedcellbiology.com

The expression level of c-myc oncogene affected by exposure to low frequency electromagnetic fields (LF-EMFs) in mouse  esenchymal stem cells (MSCs)

Yehia Mustafa, Sanaa Riad, Salah Abdel-Rahman, Maha El-Demellawy, Sarah El-Messeiry

1Department ofGenetics,Faculty ofAgriculture,AlexandriaUniversity,Alexandria, (EGYPT)
2Department ofNucleicAcidResearch,Genetic Engineering and BiotechnologyResearch Institute,City of Scientific Research andTechnologicalApplications,Alexandria, (EGYPT)
3Department ofMedical Biotechnology,Genetic Engineering and BiotechnologyResearch Institute,City of Scientific Research andTechnologicalApplications,Alexandria, (EGYPT)

Abstract

Electromagnetic fieldswhich can be generated fromhomes and workplaces appliances can lead to alterations in oncogenes which cause cancer diseases in human such as breast cancer, leukemia, nervous system tumors and lymphoma. The aim of our study is to investigate the effect of LFEMFs (50 Hz) on c-myc oncogene expression level inmouse mesenchymal stemcells (MSCs) by exposure toACelectromagnetic flux density: 0.38mT, 0.82mT,1.25mT,1.66mT,2.13mT,2.52mT,2.79mT,3.06mT,3.71mT,3.96mT, 4.08mT and 4.52mT using exposure unit. After 4 days of exposure, when initial changes in cell viability, morphology and count between exposed and unexposed cells were noted, total RNA was extracted to evaluate the expression level of c-myc oncogene by quantitative real time PCR. Results indicated that c-myc oncogene expression level began to increase with value 3.61%at 0.38mT, reaching to themaximumvalue 95.9%at 4.52mT in day 4. After 14 days of LF-EMFs exposure, c-myc gene expression level was 29.9%at 0.38mT and increased to reach themaximumvalue 98.6%at 4.52mT. These findings reveal that the effect of LF-EMFs on oncogenes expression level is very significant and deserves attention, causing harmful effects on human health and population.

Keywords:
LF-EMFs; c-myc oncogene; MSCs; EL4-Lymphoma; qRT-PCR.
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